A serial dilution is the stepwise of a in. Usually the at each step is constant, resulting in a of the in a fashion. A ten-fold serial dilution could be 1, 0.1 M, 0.01 M, 0.001 M.

1. 3oh 3
2. Colourthink

Serial dilutions are used to accurately create highly diluted solutions as well as solutions for resulting in with a. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10 0.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10 0.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution. Serial dilutions are widely used in experimental sciences, including,.

Contents.In biology and medicine In and, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. As, for instance, the number and size of that grow on an plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids (for comparing concentrations of two organisms or cell types in the sample) or wells of a given volume (for absolute concentrations), dilution can be useful for getting more manageable results. Serial dilution is also a cheaper and simpler method for preparing than.

In homeopathy. Experiments in Microbiology, Plant Pathology and Biotechnology. New Age Publishers, 2005, p. 69. Booth, C.; et al.

Methods in microbiology 35. Academic Press. P. 543. Weissmann, Gerald (2006). The FASEB Journal.

20 (11): 1755–1758. Anytrans mac. Retrieved 2008-02-01. Ernst, Edzard (November 2005). 'Is homeopathy a clinically valuable approach?'

3oh 3

Trends in Pharmacological Sciences. 26 (11): 547–548. Michael L. Bishop, Edward P. Fody, Larry E.

Clinical Chemistry: Principles, Procedures, Correlations. Lippincott Williams & Wilkins, 2004, p. 24.External links., Bates College.

Colourthink

A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step.In serial dilutions, you multiply the dilution factors for each step.The dilution factor or the dilution is the initial volume divided by the final volume.#DF = Vi/Vf#For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution,#DF = Vi/Vf# = #(1'mL')/(10'mL') = 1/10#. This is a 1:10 dilution.Example 1What is the dilution factor if you add 0.2 mL of a stock solution to 3.8 mL of diluent?#Vf# = 0.2 mL + 3.8 mL = 4.0 mL#DF = Vi/Vf# = #(0.2'mL')/(4.0'mL') = 1/20#. This is a 1:20 dilution.Example 2If you did the above dilution four times, what would be the final dilution factor?Solution 2Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume. So you multiply each successive dilution by the dilution factor.You would transfer 0.2 mL from Tube 1 to 3.8 mL of diluent in Tube 2 and mix. Then transfer 0.2 mL from Tube 2 to 3.8 mL of diluent in Tube 3 and mix. Repeat the process until you have four tubes.The dilution factor after four dilutions is#DF = 1/20 × 1/20 × 1/20 × 1/20 = 1/160000# = 1:160 000If the concentration of the original stock solution was 100 µg/µL, the concentration in Tube 4 would be100 µg/µL × #1/160000# = 6.25 × 10⁻⁴ µg/µLHope this helps.